Composite

Part:BBa_K3031020:Design

Designed by: David Doyle   Group: iGEM19_SUIS_Shanghai   (2019-10-20)


RBS+OxyR+TrcXpromoter+GFP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1917


Design Notes

Using the correct promoter sequence with two OxyR binding sites. Needed a constitutive promoter upstream of OxyR to ensure that the amount of OxyR produced is enough to interact with levels of H2O2 and positively regulate the TrxC promoter.


Source

Existing Biobricks

References